FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_GO5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_GO5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14955240
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT126350.019.5605961
CTACCCA79100.019.4667261
ACTTCGA93100.019.2853685
TCGAGAG90050.019.2389648
CGCTATC96150.019.204591
CTGATCC55950.019.1860791
GGAATTA68600.019.1476149
TAAGAAA62800.019.1437646
TACACGA90550.019.0003171
GTCTGCA108800.018.93448
CGAGAGC91550.018.9122759
GGTCTGC116250.018.877087
TCGGACA94450.018.8763168
GCGACTT93800.018.8732382
AGGACGA58600.018.8315728
ACGCACC66550.018.79079610
CTATCGG90950.018.759975
TTGACGA54450.018.70489910
GAATTAC70450.018.67461210
CGCTTCA64950.018.6621671
Produced by FastQC (version 0.11.5)