FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_GO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_GO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11253128
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA34650.020.2191241
GCGGAAG11800.019.843043
CGCTGGA33800.019.111651
GTAACTG41600.018.9620971
TTGAACG28950.018.9029221
GAAGACG9500.018.899836
AGGGTGA25700.018.7594171
GAAGCGA7700.018.5452866
TGCGGAA12650.018.5100422
GGGATGA31750.018.493021
AGGACGA14050.018.458668
TGACGGC21650.018.4359471
GACTGCG42750.018.2800161
ATTGGTG50550.018.2229291
GGGAGAT30650.018.1628991
GGCTCGA37100.018.1194841
GACTAAC32650.018.111941
TGAAGAC11550.018.0907445
GTGATAA31700.018.0847668
TTAGGCA39000.018.0177781
Produced by FastQC (version 0.11.5)