FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_ADT5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_ADT5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17140458
Sequences flagged as poor quality0
Sequence length27
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACC191350.020.0847631
GCCCATG56150.019.485492
CCCATGT56700.019.3889583
CTACCTC149000.019.386313
GGGAGAT132350.019.3324171
TACCTCA156750.019.2717844
CGTGGGA82300.019.034759
TTGAGGC73500.019.0285845
TTTACTG104550.018.9976671
TTGAACG73800.018.9717141
TCGTGGG82750.018.9693038
TGCCTGC71050.018.9601069
TGACGGC74000.018.9488261
CTGATCC85050.018.9445291
ATTGAGG76150.018.9317264
CATGGCG83750.018.8999751
TTGTGCC67900.018.8969216
ATGGCGC60500.018.8654672
GCCTGCA71250.018.86267710
GACGACG86500.018.8509649
Produced by FastQC (version 0.11.5)