FastQCFastQC Report
Wed 24 Oct 2018
HMHGCBGX7_n01_ADT3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHGCBGX7_n01_ADT3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394191
Sequences flagged as poor quality0
Sequence length27
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC20100.018.6563361
CAATGGA21150.018.2690268
CGCTGGA19800.018.2493531
ATAGACC21000.018.2069131
CTGCGGA17900.018.1912631
CGGACTG15650.018.1890131
ACTGAAC17900.018.07391
GATGAAA18950.017.9593241
CTAATGG21000.017.90681
GACTAAC16150.017.8860471
GCGCGAT15250.017.839561
ACGAGCC19400.017.8134631
GATGCTA19900.017.788161
GGATTAC23300.017.6268631
TTGGAAC18150.017.5934561
GTAACTG22500.017.553331
TTGAACG19000.017.5250761
GAATGAA17750.017.516511
TGACTAG22150.017.4513191
TACGGAT28000.017.3315811
Produced by FastQC (version 0.11.5)