FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93024714
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGATATTGCGCTCGTGTAG42910144.612767742559252No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGATATTGCGCTCGTGTAG28129343.02385664953509No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19911082.140407548041481No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGAACAGTAAGATGTGTAG4781990.5140558669172581No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGAGTTGTAGTGCGTGTAG4694390.5046390145311277No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGACACAACTTAAGTGTAG2963170.31853578179235253No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGACCAGGCACCAGTGTAG2839840.3052780146144819No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGACCAGGCACCAGTGTAG2672430.28728172171537125No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGAACAGTAAGATGTGTAG2484720.26710321302358425No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGATGATGGCTACGTGTAG2139590.23000231959863912No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGAACCACGACATGTGTAG2106480.22644305039196355No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGACACAACTTAAGTGTAG2090500.22472522731969916No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGAACCACGACATGTGTAG1967670.2115212092992836No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGAGTTGTAGTGCGTGTAG1767780.1900333711318908No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGATCACTGTCCGGTGTAG1656810.17810428312630985No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCCGTGGAGTGTAG1538070.1653399332138769No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGATCACTGTCCGGTGTAG1478300.15891475893169638No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTATCAGGGTGTAG1191840.1281207916425306No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGATGATGGCTACGTGTAG1009450.10851417398606569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)