FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n02_M11_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n02_M11_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149942352
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCGACGAACAGTAAGATGTGTAG4102930827.36338829739045No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGAACAGTAAGATGTGTAG1701706511.349071675226224No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGACCAGTAAGATGTGTAG3237250.2158996412167791No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGAACAGTAAGAGGTGTAG2405260.16041231632807787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)