FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n02_F4_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n02_F4_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92318196
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGAGTTGTAGTGCGTGTAG1451680015.7247440147119No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGAGTTGTAGTGCGTGTAG66599207.214092441754386No Hit
ACTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGTAGTGCGTGTAG1072390.11616236521779519No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGTAGTGCGTGTAG1046430.11335035186346146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)