FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_M9_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_M9_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129870779
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAATCGTATCTC59015194.544146916990465No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAATCGTATCTC31042412.390253622795317No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAATCGTATCTC5367330.4132823442908585No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAATCGTATCTC2412930.18579468134244426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)