FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_M13_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_M13_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128249602
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTAGAATTAATCTC77220086.021077554689019No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTAGAATTAATCTC74694775.824171680470401No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTAGAATTAATCTC45177253.5226035243368634No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTAGAATTAATCTC40460243.1548043322582786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)