FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_M11_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_M11_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149942352
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGCAATAATCTC2299643815.33685292598318No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGCAATAATCTC1561503210.414023650902848No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGCAATAATCTC116129897.744969213234697No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACGCAATAATCTC73915814.929615216386629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)