FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_M10_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_M10_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108520634
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAAATCTC2304380421.234490760531312No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAAATCTC1430916413.185662000463433No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAAATCTC80983377.462485890010558No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAAATCTC42480983.9145532452381357No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACAAGACTTCTGTAGAAATCTC5098200.4697908418043338No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAATCTCG4090130.37689883013400016No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAAAAATCTC3263460.30072253355983897No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACAAGACTTCTGTAGAAATCTC3099900.28565074546099684No Hit
TCTGTCTCTTATACACATCTCCGAACCCACGAGACTTCTGTAGAAATCTC2559980.23589799521444005No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAATCTCG2394120.22061426585473137No Hit
TCTGTCTCTTATACACATCTCAGAGCCCACGAGACTTCTGTAGAAATCTC2112700.1946818703620917No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAAAAATCTC1884790.1736803343777No Hit
ACTGTCTCTTATACACATCTCCGAACCCACGAGACTTCTGTAGAAATCTC1670900.1539707186008515No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAAAAATCTC1496260.13787792651487826No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTATAGAAATCTC1472150.13565622921075082No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTGTAGAATCTCG1394340.12848616420726033No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACAAGACTTCTGTAGAAATCTC1390630.12814429373864514No Hit
ACTGTCTCTTATACACATCTCAGAGCCCACGAGACTTCTGTAGAAATCTC1365970.1258719148286583No Hit
TCTGTCTCTTATACACATCTCCGAGCCCAGGAGACTTCTGTAGAAATCTC1165970.10744223997069535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)