FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_F8_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_F8_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88238133
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTCTATTAAATCTC29049013.2921152128184765No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTCTATTAAATCTC18313272.075437158218205No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTCTATTAAATCTC17739362.010396117515315No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGTCTATTAAATCTC12089851.3701389171504796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)