FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_F3_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_F3_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118907552
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTCACACGCATCTC106779818.980069659494799No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTCACACGCATCTC64352055.41193968907879No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTCACACGCATCTC26192902.2027953279199624No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTCACACGCATCTC13715061.1534221140134144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)