FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_F2_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_F2_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134651519
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTC1840706513.670150278809704No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTC109832008.156759078224733No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTC86832826.448707050976528No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTC46392583.445381110034117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)