FastQCFastQC Report
Tue 31 Oct 2023
HMHF2DRX3_n01_F1_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHF2DRX3_n01_F1_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102341916
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCGGTCAGATCTC28363372.7714323816255306No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCGGTCAGATCTC11792551.1522698089803205No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCGGTCAGATCTC8271320.8082045288266834No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCCGGTCAGATCTC3084130.3013555071609173No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)