FastQCFastQC Report
Tue 17 Oct 2023
HMHCYDRX3_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMHCYDRX3_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146154834
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG8829810.6041408113808949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATGCATTGGATGGATGCC8335600.570326671507834Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840300.2627555924698324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTCGTGCCTCCGGC1657410.11340097037091501Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)