FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30423501
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4532971.4899567278598214No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN410160.1348168312384561No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN342890.11270563502865762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA25500.012.6850369
CGGGAAT27800.010.600141
GTGCGCG45300.08.6101958
CCGGGAT49550.07.7697331
ACACGTG53400.07.65825754
GTGTCGA18100.07.6188984
CGGGGTT47900.07.541241
TAACACG52900.07.4588662
TGTTACG16109.276846E-117.3826733
CCGGAAT35450.07.3741361
CCGGCAT34200.07.36570741
AACACGT55750.07.2489243
CGAGGAT45900.07.24853231
CGGAGAT48300.07.18357131
CGAATTG19900.07.1674993
CTCGAAT32550.07.15500261
CTCGGAT40900.07.08877561
CGGAAAT35150.07.0314151
CTGGAAT124000.07.0144581
GGGGAAT81150.06.96984531
Produced by FastQC (version 0.11.5)