FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30122911
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA47100.014.126569
TCGACCG39650.010.12533226-27
ACGTGCG109250.09.9602546
CGATCCG38700.09.9385578-79
GTGCGCG81950.09.6841968
GTGTCGA36100.09.6067724
TAACACG91650.09.4841612
AACACGT102200.09.2493523
CTACCGT36300.09.1640518
GTTCGAC48050.08.99811124-25
CGGGGTT115150.08.6616721
CCGGTAT51100.08.6438741
TACCGTA37400.08.6410519
CGGAAAT67150.08.20461751
GCGTCGT196200.08.20229494-95
CCGGGAT115800.08.1208781
ACACGTG119700.08.0965094
CGACCTA17800.08.0068764
CGGGAAT63700.07.9779391
GATCGGA487350.07.96795894-95
Produced by FastQC (version 0.11.5)