FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38729239
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA57950.014.351879
CGGGAAT78650.010.2658011
TTACGAC35350.09.94648555
GTTACGA34650.09.7347464
ACGTGCG131250.09.3404176
GTGCGCG95650.09.1418188
TACGACT37750.09.0627796
CGGGGTT147350.08.8639081
CCGGGAT138700.08.5263981
CGATCCG42200.08.43888878-79
GTGTCGA44050.08.4123674
CTACCGT45550.08.3464428
CGCGAGT58000.08.19432310-11
TCGACCG43650.08.10894926-27
TGTTACG39400.08.0778323
CGCGGAT36700.08.0235821
CGGGCAT132000.07.9876991
TAACACG123450.07.96447752
AACACGT136250.07.8793093
CGGCAAT59800.07.862791
Produced by FastQC (version 0.11.5)