FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29961898
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA55900.016.5781679
GTTACGA28750.012.3934024
TAACACG103650.011.2272442
ACGTGCG129550.011.1865156
GTGCGCG89550.011.0908818
CCGGTAT61850.010.6737421
TTACGAC33350.010.5425885
GTGTCGA39000.010.4761314
AACACGT116550.010.4744883
TGTTACG32700.010.4591573
TGTCGAG43650.010.0141695
TACGACT35100.09.8820756
AATCCGA54900.09.2602515
ACACGTG133300.09.0881844
CCGGGAT126750.09.0679471
CGCGAGT55600.09.06072410-11
CGGGAAT61050.09.0243071
ATCCGAC45750.08.7240986
CGGTATT65450.08.7085932
TCGACCG43950.08.702353526-27
Produced by FastQC (version 0.11.5)