FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27920920
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA49850.017.0669
CGCGTAT4958.796633E-913.4316081
GTGTCGA33300.011.9843534
GTGCGCG82200.010.927188
ACGTGCG122500.010.6677076
TAACACG94050.010.6552362
ATCCGAC38500.010.6148126
AATCCGA48250.010.3402985
TGTCGAG40150.010.1778015
GTTACGA26100.010.0115594
AACACGT107850.09.8658433
CCGGTAT57300.09.7798581
CGATCCG35950.09.70748778-79
TCGACCG39250.09.32104226-27
TACGACT29450.08.8746646
CGGTATT62450.08.8219582
CGCGAGT52850.08.72342310-11
TGTTACG30950.08.5947593
TGTGTCG49550.08.5320363
ACACGTG125550.08.4007064
Produced by FastQC (version 0.11.5)