FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26686146
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA51450.017.2731939
GTGCGCG82400.011.99578
GTTACGA25600.011.8769254
TAACACG97800.011.0245862
ACGTGCG118250.010.8093186
GTGTCGA34400.010.6339924
TGTTACG27350.010.5943983
TTACGAC30550.010.2648215
CGATCCG40150.010.230247578-79
AACACGT109250.010.217633
TACGACT33500.09.5033596
TCGACCG43700.09.45855226-27
CCGGGAT125300.09.2485771
ACACGTG124750.09.1778414
CGCGTAT5701.0125968E-49.1654551
GTTCGAC48950.08.92948524-25
TGTCGAG42800.08.7700485
CGCGAGT56400.08.76365610-11
CTAACAC146500.08.720691
AATCCGA47500.08.7025045