FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38281539
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA72600.016.6927269
GTGTCGA49350.012.0333934
TAACACG140700.011.5112712
GTGCGCG114300.011.3505188
GTTACGA35450.011.2571464
ACGTGCG175100.011.0449096
AACACGT161300.010.5723193
TTACGAC39900.010.2412575
CGATCCG52950.010.13341978-79
TGTTACG41350.09.6497033
TGTCGAG58600.09.6488835
AATCCGA64700.09.5469975
TCGACCG59750.09.4226626-27
TACGACT42900.09.4148946
ACACGTG179550.09.3137054
CCGGGAT174200.09.0514421
CGCGAGT78000.08.77421110-11
GTTCGAC69100.08.69793824-25
CTAACAC207250.08.6621381
ATCCGAC55300.08.5926826