FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41037804
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA74350.017.7698739
GTGCGCG118850.011.5955618
TAACACG141600.011.0022522
ACGTGCG164000.010.6621426
TCGACCG55050.010.57249326-27
CGATCCG52150.010.10668278-79
CGCGTAT8056.872142E-910.0298361
GTTACGA37500.010.0082444
AACACGT162850.09.9758293
ATCCGAC54900.09.6070746
TTACGAC41600.09.5940285
TACGACT43250.09.3384186
CGCGAGT76200.09.32442610-11
GTTCGAC66950.09.22573424-25
ACACGTG180650.08.9939444
CCGGTAT82500.08.8655881
TGTTACG42750.08.6670073
AATCCGA67550.08.581235
CGGGAAT81750.08.5402461
CGAGTCG84850.08.40210212-13
Produced by FastQC (version 0.11.5)