FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23490501
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA41650.016.8880889
GTGCGCG71950.010.30365858
TAACACG80900.010.1571582
ACGTGCG97350.09.7623156
CGATCCG29650.09.60862178-79
TCGACCG32050.09.48741226-27
GTGTCGA27400.09.1894344
AACACGT92000.09.0873533
CGGGAAT47250.08.9462221
GTTACGA19350.08.8386314
CCGGGAT100800.08.764031
GTTCGAC39500.08.66040524-25
CGCGAGT45200.08.41217810-11
ACACGTG104150.08.3474784
GATCGGT59650.08.2840875
TACGACT25200.08.1082426
CTAACAC126200.08.0162581
TTACGAC23150.07.79927735
CCGGCAT56500.07.73376271
CGCGATT8601.7018738E-57.7318081
Produced by FastQC (version 0.11.5)