FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31495856
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA55600.012.479479
ATCCGAC42550.09.6036936
GTGTCGA38850.09.4160044
GTGCGCG87550.09.3359678
GTTACGA29150.09.2897324
AATCCGA53900.09.1677175
ACGTGCG133950.09.04558856
TAACACG103250.08.7862432
TTACGAC31250.08.6664545
TCGACCG43700.08.64330626-27
TGTTACG30300.08.4657533
AACACGT118150.08.4028593
CGATCCG39300.08.39729578-79
TACGACG39500.08.3017599
CCGGGAT128750.08.263031
GATCGGA386900.08.15348194-95
CCGGTAT59650.08.121351
GTTCGAC48600.08.11421924-25
TACGACT36400.08.0933766
CGGTATT62250.08.0877272
Produced by FastQC (version 0.11.5)