FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40305811
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA76500.015.53121959
TAACACG138350.010.7801432
ACGTGCG172250.010.4551656
GTGCGCG119800.010.2740558
AACACGT157700.09.8197123
GTGTCGA53600.09.48393254
TCGACCG55650.08.79366326-27
CGATCCG54000.08.57335178-79
ACACGTG179650.08.4359284
CGGGAAT83800.08.3882361
GTTACGA36900.08.36866954
CGCGAGT80300.08.28626510-11
CGTTCGA57500.08.2642327
CTCGGAT143750.08.2600971
CGCGGAT44900.08.2508981
CTAACAC208000.08.2432181
TGTCGAG62500.08.2105545
CCGGTAT84500.08.2063331
CGGGGTT146550.08.1022781
CCGGGAT165800.07.96366931
Produced by FastQC (version 0.11.5)