FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24538315
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA47150.014.5150439
GTGTCGA30400.010.9394924
TAACACG87950.010.5852422
CCGGTAT50150.010.3224751
GTGCGCG75150.010.1813258
AACACGT95000.010.0504563
ACGTGCG104050.09.910026
TGTCGAG34500.09.5031065
CGATCCG31350.09.4658578-79
CGGGAAT49550.09.3931391
TAGCCGA34900.09.2602149
GTTACGA21650.09.2164584
CGGGGTT90150.09.0086291
TCGACCG33500.08.86419626-27
ACACGTG108200.08.8255444
CGCGAGT46650.08.55812210-11
CCGGGAT99350.08.5568421
CGCGATT8551.7601815E-68.3320811
CAGACGT73500.08.2724853
TGTTACG24450.08.159883
Produced by FastQC (version 0.11.5)