FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23883948
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA43500.013.0009439
GTGTCGA31650.012.4588944
GTTACGA23850.011.5535314
TGTTACG24700.010.9622633
TTACGAC24450.010.8827335
TACGACT26450.010.2398686
GTGCGCG67900.09.5883398
TCGACCG32900.09.3868326-27
ATCCGAC34700.09.3116066
TGTCGAG37800.09.3018375
ACGTGCG100300.09.1906446
TAACACG81850.09.1105212
CGGGGTT79050.08.9518911
AATCCGA38450.08.8974385
CGATCCG32000.08.60621978-79
AACACGT93250.08.354443
GTTCGAC41000.08.28583824-25
TCGCGAA20700.08.26194332-33
CTACCGT27650.08.249718
CGGGCAT85450.08.1702561
Produced by FastQC (version 0.11.5)