FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42317779
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA73500.012.802499
GTTACGA38350.010.0340194
CGATCCG54100.09.78628278-79
TCGACCG58500.09.38057526-27
ATCCGAC58500.09.259636
TAACACG140350.09.20509052
ACGTGCG164800.09.1400116
GTGTCGA52700.08.92441754
GTTCGAC68200.08.91736724-25
TGTTACG43500.08.7358193
GTGCGCG121150.08.5903448
TACGACT46100.08.5550366
CGGGGTT154600.08.4789871
CCGGGAT168900.08.4641081
AATCCGA72200.08.4234745
GCGTCGT271700.08.35174694-95
GATCGGA696300.08.34154694-95
CCGGTAT79200.08.3355451
AACACGT160200.08.30233
CGTTCGA62450.08.29397
Produced by FastQC (version 0.11.5)