FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33717262
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA60100.014.4718169
GTTACGA34400.010.4958374
TTACGAC35900.010.3235225
TAACACG107250.010.00865652
ACGTGCG129500.09.8705766
GTGCGCG100700.09.5804368
TACGACT39200.08.9702726
AACACGT124050.08.960393
TCGACCG49000.08.92039426-27
TGTTACG38400.08.9065413
CCGGGAT141550.08.8577851
CCGGTAT69400.08.3489421
CGCGAGT59550.08.26087910-11
CAAACGA103800.08.2417919
TAATCGA80450.08.2082534
CGATCCG47150.08.15720478-79
CTAATCG73000.08.1338273
GTTCGAC55450.07.840161324-25
CGCGGAT38850.07.8238421
CGGCAAT55350.07.80827141
Produced by FastQC (version 0.11.5)