FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26236326
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA51300.013.2473749
GTTACGA23850.011.3540094
TTACGAC29350.09.71297555
TGTTACG29750.08.9415433
CTACCGT32550.08.9056568
ACGTGCG105350.08.8401176
GTGCGCG83400.08.7748918
TAACACG88400.08.7042272
TACGACT32800.08.6918846
ATCCGAC38400.08.6617046
TCGACCG36700.08.47917826-27
AATCCGA45500.08.4582945
AACACGT97550.08.4242313
CGATCCG36350.08.22993578-79
CGACGAT21950.08.2222851
GATCGGA231750.08.15322894-95
CGCGTAT7151.333212E-47.9711081
AGATCGG272150.07.85873694-95
CCGGTAT54950.07.5196051
CCGGGAT109250.07.4773981