FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n02_ALm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n02_ALm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26332531
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA47450.011.1171859
GTTACGA25700.09.9821534
CGATCCG36150.09.45744978-79
CCGGTAT50650.09.3771361
AATCCGA43750.09.3397835
ATCCGAC33950.09.0973126
ACGTGCG101450.08.7584876
CCGGGAT105050.08.7259131
TTACGAC28000.08.6542325
TACGACT28700.08.6091636
TAACACG86100.08.4403612
TCGACCG40600.08.42552226-27
CGGGGTT97050.08.4174891
CGGTATT53250.08.11698152
CGGGAAT53300.08.1089351
CTACCGT31250.08.0595348
TGTTACG30450.07.9559643
AACACGT99450.07.9289043
GTTCGAC46000.07.849635624-25
TACGACG39500.07.8203179
Produced by FastQC (version 0.11.5)