FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30423501
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3617631.1890906309566411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT59700.025.65750344-45
AGAGCAC241500.023.4878928
TCGGAAG242550.022.9149513
GATCGGA242400.022.1756531
ATCGGAA254000.022.1058772
CGTCTGA128400.021.60041216-17
ATGCCGT79450.021.55090948-49
CGGAAGA264600.021.4531944
ACGTCTG130250.020.99777414-15
CACGTCT135400.020.65420714-15
TCTCGTA74700.020.60041842-43
ACACGTC135100.020.55611612-13
GTCACGA53500.020.3399628-29
CACACGT148450.019.07464612-13
TATCTCG38250.017.72929840-41
ATCTCGT85350.017.33536542-43
GCACACG175750.015.94908210-11
GAGCACA381350.015.4956729
TGCGCGA29200.015.09273059
ACGAGAT77000.014.96115132-33
Produced by FastQC (version 0.11.5)