FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30122911
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT962850.3196404225341966TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG153050.039.69472544-45
CGTATGC159600.038.94353546-47
CTCGTAT147400.037.703744-45
TATGCCG165800.037.2867248-49
GTCACGA198000.032.22910728-29
TCTCGTA173100.030.61033842-43
ATGCCGT201050.030.53662148-49
AGTCACG217650.029.67946228-29
GTATGCC213350.029.12122546-47
ATCTCGT184200.028.9075742-43
GCCGTCT208400.028.44533550-51
TCACGAG232600.028.16015230-31
ACGAGAT234400.027.53874432-33
CACGAGA239250.026.8711830-31
TGCCGTC231600.026.45728950-51
CATCTCG201050.026.1303940-41
GAGCACA545250.024.0951379
CCGTCTT252300.023.58056852-53
CGAGATT279900.022.9263432-33
AGAGCAC581200.022.5638758
Produced by FastQC (version 0.11.5)