FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38729239
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT929210.2399246729325097TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC157100.037.76402746-47
TCGTATG153550.037.5854244-45
CTCGTAT151350.035.68378444-45
TATGCCG169250.034.74435848-49
GTCACGA196550.030.73910728-29
AGTCACG220000.028.18586728-29
ATGCCGT207000.027.81151248-49
TCTCGTA186150.027.3414142-43
GTATGCC221450.026.42573546-47
ATCTCGT198250.026.12788442-43
TCACGAG245550.025.45637330-31
ACGAGAT243100.025.38083332-33
GCCGTCT223700.024.96018850-51
TGCCGTC240000.024.2842450-51
CACGAGA250900.024.26043130-31
CATCTCG209850.024.1176940-41
GAGCACA594450.021.1901199
TCGGAAG585350.020.8716323
CCGTCTT274150.020.74804152-53
ATCGGAA588350.020.741042
Produced by FastQC (version 0.11.5)