FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29961898
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT699560.23348320590371144TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG110100.037.6636944-45
CGTATGC113600.037.02594846-47
CTCGTAT108800.035.6688544-45
TATGCCG128500.033.00990348-49
GTCACGA140550.030.98944728-29
AGTCACG150900.029.16295228-29
ATGCCGT157450.026.6387648-49
TCACGAG168000.026.44932230-31
GTATGCC167950.025.01576446-47
TCTCGTA146500.024.8524442-43
GCCGTCT172050.023.93644550-51
CACGAGA183750.023.88496830-31
TCGGAAG374100.023.4775683
ATCGGAA374100.023.2492472
GATCGGA371850.023.1960641
ATCTCGT164050.022.70044342-43
ACGAGAT197650.022.3856332-33
GAGCACA405100.022.2069959
TGCCGTC194300.022.01436450-51
CATCTCG166750.021.89116740-41
Produced by FastQC (version 0.11.5)