FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27920920
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT509320.18241519262259268TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG88850.035.9524744-45
CGTATGC92200.035.3159146-47
CTCGTAT88150.033.94783444-45
TATGCCG108300.029.80267548-49
GTCACGA115600.029.11108628-29
AGTCACG123950.027.57151428-29
ATGCCGT130300.024.42444248-49
TCACGAG141650.024.37795830-31
TCTCGTA127250.022.62081742-43
GTATGCC145050.022.46468246-47
GCCGTCT141500.021.97084250-51
CACGAGA156250.021.65929630-31
ACGAGAT162300.020.93982132-33
GATCGGA319050.020.8526271
ATCTCGT139200.020.67887342-43
ATCGGAA325100.020.6163832
TCGGAAG328400.020.5247843
TGCCGTC159550.020.12534550-51
TGCGCGA50100.019.7196889
CATCTCG143100.019.71688740-41
Produced by FastQC (version 0.11.5)