FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26686146
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT646170.24213687506618606TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG109600.037.90049444-45
CGTATGC113450.037.76574746-47
CTCGTAT107700.035.92286744-45
TATGCCG125350.033.66897648-49
GTCACGA144150.030.42988828-29
AGTCACG153800.029.23091328-29
TCACGAG166500.027.27240430-31
ATGCCGT154050.026.85673948-49
GTATGCC160200.026.19630846-47
TCTCGTA142850.025.03845442-43
GCCGTCT160500.024.75632350-51
ATCTCGT152350.024.28777742-43
CACGAGA183150.024.07990530-31
ACGAGAT186300.023.97888232-33
TGCCGTC183600.022.65054750-51
CATCTCG158950.022.51715340-41
GAGCACA406150.022.1263799
TCGGAAG403200.021.8537463
ATCGGAA404800.021.6266372
GATCGGA402100.021.5796011
Produced by FastQC (version 0.11.5)