FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38281539
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1147350.29971365571274444TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG183450.039.0200844-45
CGTATGC189150.038.50969346-47
CTCGTAT178300.037.32324644-45
TATGCCG205950.035.1146648-49
GTCACGA247700.030.49885728-29
ATGCCGT243850.029.26743548-49
AGTCACG267850.028.5591328-29
GTATGCC256050.028.2346646-47
TCACGAG277650.027.69670930-31
GCCGTCT256450.027.00513350-51
TCTCGTA233150.026.75991842-43
TGCCGTC282100.025.58521850-51
ATCTCGT248950.025.50039742-43
CACGAGA304250.024.9161730-31
ACGAGAT312150.024.51386532-33
CATCTCG256000.024.31562640-41
CCGTCTT328950.021.45750852-53
GAGCACA804700.019.0770519
GATTCCA396450.019.07390636-37
CGTCTTC367950.019.04762352-53
Produced by FastQC (version 0.11.5)