FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41037804
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT749020.18251951298368696TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC134200.035.09395646-47
TCGTATG132000.034.76132644-45
CTCGTAT128950.033.2260244-45
TATGCCG154900.030.31224848-49
GTCACGA172650.027.81372828-29
AGTCACG190500.025.66882528-29
ATGCCGT192000.024.306648-49
TCACGAG211000.023.4114730-31
TCTCGTA175450.023.2151842-43
GTATGCC204650.023.0594146-47
GCCGTCT211000.021.60004250-51
ATCTCGT195150.021.27326642-43
CACGAGA231550.020.98498530-31
ACGAGAT233850.020.89037732-33
TGCCGTC234500.020.11400250-51
TGCGCGA76250.019.684349
CATCTCG206800.019.48911540-41
GAGCACA586650.017.1482779
CGTCTGA292700.017.06318516-17
TCGGAAG579550.016.8839783
Produced by FastQC (version 0.11.5)