FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23490501
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT446310.18999594772372033TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG74950.036.1240144-45
CGTATGC77400.035.87036546-47
CTCGTAT74700.033.95575344-45
TATGCCG89400.031.02898648-49
GTCACGA94900.029.95535728-29
GATCGGA208150.027.23791
AGTCACG107000.027.10056928-29
ATCGGAA217200.026.1997182
TCGGAAG223450.025.5942843
TCACGAG119450.024.49490230-31
ATGCCGT112250.024.12019248-49
GTATGCC118250.023.21768846-47
TCTCGTA104450.023.07912842-43
ACACGTC127950.022.2732612-13
ACGAGAT130550.022.24854932-33
CACGAGA130150.022.11615430-31
GCCGTCT122550.021.5115650-51
GAGCACA273450.021.4864469
CGGAAGA273450.021.24444
ATCTCGT117150.021.08399242-43
Produced by FastQC (version 0.11.5)