FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31495856
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT730200.23184002365263545TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC115150.036.67143646-47
TCGTATG112650.036.62090744-45
CTCGTAT116750.034.4194544-45
TATGCCG127400.032.8472248-49
TATCTCG123350.031.9808440-41
ATGCCGT160250.025.68400248-49
TCTCGTA155200.025.509642-43
GTATGCC170400.024.5163846-47
ATCTCGT169700.024.19763642-43
GTCACCG182550.023.69036128-29
CACCGCT188700.022.86809730-31
GCCGTCT175550.022.79620750-51
ACCGCTC193150.022.78393632-33
CGCTCAT205650.021.49165334-35
TGCCGTC195800.021.2876650-51
CCGCTCA205350.021.09493332-33
TCACCGC231050.019.22129630-31
GAGCACA472250.019.09979
TCGGAAG465300.019.0695363
CATTATC218400.018.97589338-39
Produced by FastQC (version 0.11.5)