FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40305811
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT714730.17732678794132192TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC120500.034.1960546-47
TCGTATG118700.034.07433344-45
CTCGTAT119950.032.25405544-45
TATGCCG136400.030.0880648-49
TATCTCG132200.028.6182840-41
TCTCGTA176300.021.67529742-43
GTATGCC189200.021.65366746-47
ATGCCGT188300.021.60587548-49
ATCTCGT193100.020.57666642-43
GTCACCG212950.020.00726928-29
GCCGTCT198550.019.94019750-51
ACCGCTC219100.019.73857132-33
CACCGCT220550.019.2749330-31
CGCTCAT240050.018.17425734-35
TGCCGTC227450.018.11662550-51
CCGCTCA240850.017.65039832-33
TGCGCGA80600.016.0289179
TCACCGC273250.016.02682730-31
CATTATC253650.015.89871338-39
GAGCACA576200.015.653889
Produced by FastQC (version 0.11.5)