FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24538315
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT474000.19316729775455244TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC75550.035.71142646-47
TCGTATG76200.034.6586644-45
CTCGTAT76800.033.55293744-45
TATGCCG88850.030.33906748-49
TATCTCG84100.029.9908240-41
ATGCCGT116850.022.72358548-49
TCTCGTA111750.022.57020242-43
GTATGCC119750.022.31209446-47
GTCACCG127650.021.58149328-29
GCCGTCT122050.021.23001550-51
ATCTCGT125700.021.12345742-43
ACCGCTC133450.020.82162932-33
CACCGCT135350.020.21346330-31
TGCCGTC135900.019.940250-51
CGCTCAT145650.019.07781234-35
CCGCTCA148700.018.4466932-33
TCGGAAG309750.018.0035843
ATCGGAA310400.017.9200482
GATCGGA307850.017.6752621
CGTCTGA166750.017.34703816-17
Produced by FastQC (version 0.11.5)