FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23883948
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT469150.19642899909177494TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG78800.036.5897444-45
CGTATGC81050.036.1600546-47
CTCGTAT80800.034.1555844-45
TATGCCG89250.032.65154348-49
TATCTCG87450.031.42231440-41
ATGCCGT117600.024.21470648-49
GTATGCC121200.024.08330746-47
TCTCGTA115250.023.78101542-43
GTCACCG132700.022.96157628-29
ATCTCGT128600.022.12490342-43
GCCGTCT127000.021.9548250-51
CACCGCT137300.021.89846430-31
ACCGCTC140700.021.60569432-33
CGCTCAT151150.020.34784934-35
TGCCGTC143300.020.03759650-51
CCGCTCA154300.019.74754332-33
TCACCGC167300.018.44011730-31
GAGCACA344400.017.9839469
TCGGAAG347950.017.5422363
CATTATC165500.017.49322338-39
Produced by FastQC (version 0.11.5)