FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42317779
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT812600.19202331010802812TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC137700.034.6850546-47
TCGTATG136000.034.6296644-45
CTCGTAT139950.032.02310644-45
TATGCCG153300.031.06252148-49
TATCTCG147000.029.98622140-41
TCTCGTA193100.022.99974442-43
GTATGCC210050.022.71545646-47
ATGCCGT207850.022.6016848-49
ATCTCGT211950.021.58174542-43
GCCGTCT212450.021.31851250-51
GTCACCG237050.020.90873128-29
ACCGCTC244800.020.35371232-33
CACCGCT245450.020.19330830-31
CGCTCAT265650.018.95307534-35
CCGCTCA259550.018.93167532-33
TGCCGTC253450.018.6195250-51
TCACCGC288200.017.58524530-31
CATTATC280050.016.6558538-39
CCGTCTT289250.015.93730352-53
TGCGCGA78300.015.8326259
Produced by FastQC (version 0.11.5)