FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33717262
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT573410.17006422407608304TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG98350.034.0736744-45
CGTATGC102450.033.42868446-47
CTCGTAT101550.032.1112344-45
TATGCCG112550.030.2600748-49
TATCTCG109600.029.29721340-41
TCTCGTA145100.022.06404942-43
ATGCCGT155350.021.63273648-49
GTATGCC161050.021.01455146-47
ATCTCGT163200.020.54836742-43
GTCACCG176100.020.202128-29
GCCGTCT164800.019.94555550-51
ACCGCTC182350.019.69220232-33
CACCGCT180950.019.63447830-31
CGCTCAT197850.018.23365834-35
TGCCGTC192400.018.01015550-51
CCGCTCA199300.017.80293332-33
TGCGCGA61100.016.6358269
GAGCACA444000.016.6348639
ACACGTC216200.016.46598412-13
GATCGGA427550.016.4281751
Produced by FastQC (version 0.11.5)