FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26236326
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT586600.22358313431537635TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG91450.036.2552244-45
CGTATGC93850.035.8594146-47
CTCGTAT95650.033.89350544-45
TATGCCG105150.031.89282248-49
TATCTCG100650.031.64326340-41
TCTCGTA131700.024.54371642-43
ATGCCGT138300.024.00780948-49
ATCTCGT139900.023.68233142-43
GTATGCC142700.023.3507746-47
GTCACCG152950.022.70085728-29
ACCGCTC154000.022.6387832-33
GCCGTCT144800.022.43794350-51
CACCGCT155650.022.4140330-31
GATCGGA309750.022.3521461
ATCGGAA316550.022.0285032
TCGGAAG322400.021.7316783
CGCTCAT166750.021.0931134-35
GAGCACA347750.020.6653759
CCGCTCA169250.020.51475532-33
TGCCGTC168550.020.17807650-51
Produced by FastQC (version 0.11.5)