FastQCFastQC Report
Fri 12 Oct 2018
HMGMVBGX7_n01_ALm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGMVBGX7_n01_ALm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26332531
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT567450.2154939075169037TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG88100.035.2876744-45
CGTATGC91300.035.09137746-47
CTCGTAT88900.033.66107644-45
TATGCCG99150.032.36105748-49
TATCTCG97800.030.42762840-41
TCTCGTA122550.024.18568642-43
ATGCCGT130550.023.97720748-49
GTATGCC133700.023.9096346-47
GCCGTCT136900.022.43139850-51
GTCACCG148900.022.3135928-29
ATCTCGT138400.022.29103542-43
CACCGCT153500.021.8462930-31
ACCGCTC157550.021.45056332-33
TGCCGTC156400.020.60650650-51
CGCTCAT166400.020.40964134-35
CCGCTCA165450.020.1966632-33
GAGCACA368500.018.9604179
GATCGGA354950.018.9051551
TCGGAAG359700.018.8577653
TCACCGC180850.018.83137530-31
Produced by FastQC (version 0.11.5)